NDLI: Analysis of Gel Electrophoresis Images

Content Provider	IEEE Xplore Digital Library
Author	Akhter, N. Khan, A.R. Talib, Y. Shadab, S. Patel, R.
Copyright Year	2008
Description	Author affiliation: Dept. of M. Sci Comput. Sci., Maulana Azad PG & Res. Centre, New Delhi (Akhter, N.)
Abstract	Electrophoregrams generated after the electrophoresis experiment can be evaluated by eye, using simple measurement tools. A basic strip or spot densitometer and a ruler can provide data that are adequate for some purposes. More detailed analysis requires more sophisticated measurement tools. We may need to know the molecular weights and proportional purities or abundances of various compounds distributed across a lane. In these sorts of quantitative applications, image analysis offers many advantages.In this paper we present a computerized image analysis of gel electrophoresis. Measurement made on image is more accurate then those made on gel itself. Image analysis is by far the quickest way to obtain quantitative data from gels. Digitized specimens provide a permanent archive that can replace tedious photo documentation. Corrections for warpage and background artifacts can be applied to the gel images before measurement. Image analysis will provide both graphical and pictorial views during analysis. Analysis using multiple views improves the accuracy with which bands are defined. Image analysis is inherently flexible. This will allow a laboratory to deal efficiently with different types of gel specimens.
Starting Page	106
Ending Page	109
File Size	329011
Page Count	4
File Format	PDF
ISBN	9780769532677
DOI	10.1109/ICETET.2008.132
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-07-16
Publisher Place	India
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Proteins computational biology Image analysis DNA size Image processing Gel electrophoresis DNA Size measurement Distance measurement Biochemistry molecular weight
Content Type	Text
Resource Type	Article

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