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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Yanlong Yang Xing Zhou Runze Li Tong Peng Ming Lei Di Wu Baoli Yao Van Horn, M. Xun Chen Ye Tong |
| Copyright Year | 2015 |
| Description | Author affiliation: State Key Lab. of Transient Opt. & Photonics, Xi'an Inst. of Opt. & Precision Mech., Xi'an, China (Yanlong Yang; Xing Zhou; Runze Li; Tong Peng; Ming Lei; Di Wu; Baoli Yao) || Dept. of Bioeng., Clemson Univ., Charleston, SC, USA (Van Horn, M.; Xun Chen; Ye Tong) |
| Abstract | Bessel beams have been used in many applications due to their unique optical properties of maintaining their intensity profiles unchanged during propagation. In imaging applications, Bessel beams have been successfully used to provide extended focuses for volumetric imaging and uniformed illumination plane in light-sheet microscopy. Coupled with two-photon excitation, Bessel beams have been successfully used in realizing fluorescence projected volumetric imaging. We demonstrated previously a stereoscopic solution-two-photon fluorescence stereomicroscopy (TPFSM)-for recovering the depth information in volumetric imaging with Bessel beams. In TPFSM, tilted Bessel beams were used to generate stereoscopic images on a laser scanning two-photon fluorescence microscope; upon post image processing we could successfully provide 3D perception of acquired volume images by wearing anaglyph 3D glasses. However, tilted Bessel beams were generated by shifting either an axicon or an objective laterally; the slow imaging speed and severe aberrations made it hard to use in real-time volume imaging. In this article, we report recent improvements of TPFSM with newly designed scanner and imaging software, which allows 3D stereoscopic imaging without moving any of the optical components on the setup. This improvement has dramatically improved focusing qualities and imaging speed so that the TPFSM can be performed potentially in real-time to provide 3D visualization in scattering media without post image processing. |
| Starting Page | 1 |
| Ending Page | 3 |
| File Size | 344794 |
| Page Count | 3 |
| File Format | |
| ISBN | 9781467379441 |
| ISSN | 21668892 |
| DOI | 10.1109/OECC.2015.7340281 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2015-06-28 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Integrated optics Three-dimensional displays two photon fluorescence Bessel beams extended depth of field volumetric imaging Fluorescence Optical imaging Parallax microscopy Image reconstruction |
| Content Type | Text |
| Resource Type | Article |
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