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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Tingting Yu Jianru Wang Yisong Qi Dan Zhu |
| Copyright Year | 2015 |
| Description | Author affiliation: Dept. of Biomed. Eng., Huazhong Univ. of Sci. & Technol., Wuhan, China (Tingting Yu; Jianru Wang; Yisong Qi; Dan Zhu) |
| Abstract | The rise of various optical clearing methods provides a great potential for imaging deep inside tissues by combining with multiple-labelling and microscopic imaging techniques. However, these methods had certain weaknesses, including toxicity of reagents, tissue deformation, fluorescence quenching, and complexity of implementation. In this study, we developed simple and rapid clearing agents for improving optical imaging depth by observing the rapid clearing process and obtaining the fluorescence images. After brain slices were immersed with sorbitol, sucrose and fructose for 1 or 2 min, respectively, the imaging depth can be enhanced rapidly with good transparency and fine fluorescence preservation for both genetically encoded green fluorescent protein and prodium iodide nuclear dye. This work provide a simple and rapid method for improving optical imaging depth. |
| Starting Page | 1 |
| Ending Page | 3 |
| File Size | 420694 |
| Page Count | 3 |
| File Format | |
| ISBN | 9781467379441 |
| ISSN | 21668892 |
| DOI | 10.1109/OECC.2015.7340074 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2015-06-28 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Optical fibers optical clearing method Fluorescence Optical imaging simple and rapid Optical refraction Optical scattering green fluorescent protein Sugar prodium podide imaging depth |
| Content Type | Text |
| Resource Type | Article |
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