NDLI: Development of a quantifiable optical reader for lateral flow immunoassay

Content Provider	IEEE Xplore Digital Library
Author	Yang, Yongliang Gu, Yongbin Ge, Shengxiang Tang, Zhanghong Qiu, Xianbo
Copyright Year	2015
Description	Author affiliation: College of Material Science and Engineering, Beijing University of Technology, Beijing, China (Tang, Zhanghong) \|\| College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, China (Yang, Yongliang; Gu, Yongbin; Qiu, Xianbo) \|\| National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen, China (Ge, Shengxiang)
Abstract	An optimized optical reader was developed for quantitative fluorescence detection of lateral flow immunoassay in point-of-care test (POCT). Different from existing readers for qualitative test, the developed reader is able to perform quantitative test with a line-shaped excitation beam to obtain the distribution of fluorescence signal intensity along the lateral flow strip. To reduce system complexity, a custom Y-shaped optical fiber, instead of one set of optical lenses, was adopted to couple the excitation and emission channels. A high power Light Emitting Diode (LED) with a specific wavelength interfaced with one of the two split ends of the optical fiber for excitation, while a highly sensitive photodiode with the other split end for detection. A linear stage with high resolution, which was driven by a step motor, was incorporated to facilitate strip scanning. Considering the inhomogeneous nature of lateral flow immunoassay, an optimized global searching algorithm was developed to identify test and control peaks and their boundaries, which further improves reader's sensitivity and specificity. Finally, a calibration model was built into the reader to report the concentration of the test sample. As an example, quantitative CRP (C — reactive protein) test was performed on this reader achieving a reasonable clinical diagnostics range from 1 μg/mL to 200 μg/mL. With its machine reading function, simplicity and relatively low cost, the optical reader could play an important role in POCT.
Starting Page	344
Ending Page	349
File Size	757742
Page Count	6
File Format	PDF
e-ISBN	9781509000227
DOI	10.1109/BMEI.2015.7401527
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2015-10-14
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Optical fibers Strips Optical fiber sensors Lateral Flow Immunoassay Point-of-Care Test (POCT) Fluorescence Quantitative Detection CRP (C — reactive protein) Global Searching Algorithm Optical Reader Immune system
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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