NDLI: Endostar down-regulates HIF-1 and VEGF expression and enhances the radioresponse of lung cancer

Content Provider	IEEE Xplore Digital Library
Author	Wei Ge Ling Zhang Ke Hu YanYan Zhang ChangHu Li Du He ZhenYu Zhao JinZhong Zhang JunJian Deng XiMing Xu
Copyright Year	2010
Description	Author affiliation: Cancer Center, Renmin Hospital of Wuhan University, China (Wei Ge; Ling Zhang; Ke Hu; YanYan Zhang; ChangHu Li; Du He; ZhenYu Zhao; JinZhong Zhang; JunJian Deng; XiMing Xu)
Abstract	Hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) play important roles in tumor angiogenesis. We examined the effect of endostar, an inhibitor of angiogenesis on the expression of HIF-1 and VEGF and on angiogenesis and radio sensitization. Flow cytometry (FCM) was used to evaluate the cell cycle phase distribution affected by endostar. The cell growth inhibiting rate was detected by methyl thiazolyl tetrazolium (MTT). Hoechst staining displayed the apoptotic cells, the expression of HIF-1 and VEGF was determined by enzyme linked immunosorbent assay (Elisa). To explore the anti-tumor efficacy in vivo, Lewis lung cancer models were established in the hind limb of female C57BL/6J mice by subcutaneous transplantation. We calculated the tumor inhibitory rate and detected the microvessel density (MVD) and VEGF expression by Immunohistochemistry. Whether in vitro or in vivo, endostar in combination with radiotherapy (RT) had the most significant inhibitory effect. Endostar had the function of block cell periods on A549 and stopped the cell cycle at G2/M and S periods. It also decreased the expression of HIF-1 and VEGF induced by radiotherapy. Endostar combined with irradiation had synergistic anti-tumor effect. And the mechanism may be that endostar can arrest the cell cycle at stage of G2/M and S, thereby, inhibiting the proliferation of tumor cells. In addition, the inhibition of HIF-1 and VEGF which play important role in resistant of radiotherapy may contribute to the synergistic effect.
Starting Page	1344
Ending Page	1349
File Size	389514
Page Count	6
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639371
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	lung cancer Radiation effects HIF-1 Lungs Humans VEGF Mice endostar radioresponse In vitro Tumors Cancer
Content Type	Text
Resource Type	Article

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