NDLI: Comparison of Gene Expression Effects in Liver Tissue and Primary Hepatocyte Cell Cultures After Exposure to Hexahydro-1, 3, 5Trinitro-1, 3, 5-Triazine

Content Provider	IEEE Xplore Digital Library
Author	Perkins, E.J. Wenjun Bao Xin Guan Choo-Yaw Ang Wolflnger, R.D. Tzu-MIng Chu Ping Gong Meyer, S.A. Inouye, L.
Copyright Year	2006
Description	Author affiliation: US Army Engineer Res. & Dev. Center, Vicksburg, MS (Perkins, E.J.)
Abstract	We examined Sprague-Dawley rat liver tissue and primary hepatocyte cells to understand how they compared in gene expression responses to hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). Gene expression was analyzed in primary cell cultures exposed to 7.5, 15, or 30 mg/L RDX for 24 and 48 hours. Cells were compared to liver from rats 24 hours after exposure to 12, 24, or 48 mg/Kg RDX. Similarity of present/absent calls to liver in control exposures was 88% at 24 hrs and 79% at 48 hrs. Significant changes were identified using mixed-model analysis of variance. Up to 6.4% of differentially expressed genes present in liver were differentially expressed in 24 hr cells and less than 5.5% in 48 hr cells. Of genes with identifiable KEGG pathways, 24 hr cells 78.4% similar to liver pathways and 48 hr cells were 44.3% similar. These data suggest care should be taken extrapolating effects from in vitro to in vivo levels
Sponsorship	STEMES/IMSCCS Conference, USA The University of Iowa, USA Zhejing Univ., China Harbin Eng. Univ., China
Starting Page	164
Ending Page	170
File Size	275055
Page Count	7
File Format	PDF
ISBN	0769525814
DOI	10.1109/IMSCCS.2006.48
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-06-20
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	In vivo Animals Liver Rats Water pollution Gene expression Synthetic aperture sonar In vitro Chemicals Research and development
Content Type	Text
Resource Type	Article

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Comparison of transcriptional responses in liver tissue and primary hepatocyte cell cultures after exposure to hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine

Comparison of transcriptional responses in liver tissue and primary hepatocyte cell cultures after exposure to hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine

Hexahydro-1 , 3 , 5-trinitro-1 , 3 , 5-triazine Mineralization by Zerovalent Iron and Mixed Anaerobic Cultures

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Comparison of Gene Expression Effects in Liver Tissue and Primary Hepatocyte Cell Cultures After Exposure to Hexahydro-1, 3, 5Trinitro-1, 3, 5-Triazine

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