NDLI: Acoustic radiation force for vascular stem cell therapy: An in vitro validation

Content Provider	IEEE Xplore Digital Library
Author	Kaya, M. Toma, C. Jianjun Wang Grata, M. Huili Fu Villanueva, F.S. Xucai Chen
Copyright Year	2011
Description	Author affiliation: Center for Ultrasound Mol. Imaging & Therapeutics, Univ. of Pittsburgh, Pittsburgh, PA, USA (Kaya, M.; Toma, C.; Jianjun Wang; Grata, M.; Huili Fu; Villanueva, F.S.; Xucai Chen)
Abstract	Coronary stent placement and angioplasty may lead to vascular injury such as postprocedural stent thrombosis and restenosis. Mesenchymal stem cells (MSCs) have been reported to participate in effective restoration of functional endothelium following vascular injury. Our group has recently investigated a new technique for the delivery of MSCs to a site of arterial injury which involved surface-coating MSCs with cationic lipid microbubbles (MB) and directing them to target areas using acoustic radiation force (ARF). The objective of this study was to characterize ultrasound parameters for effective MSC delivery through in vitro experiments in which MB-labeled MSCs flowing through a phantom vessel were radially displaced towards the vessel wall using ARF applied with an intravascular ultrasound catheter driven at varying acoustic pressures and duty cycles. Experimental data indicated that MSC radial velocity was linearly related to the time-averaged ultrasound intensity up to 0.83 $W/cm^{2}.$ Experimental data agreed with model predictions only up to this intensity level possibly due to MB destruction before the MB-MSC complexes reached the target area at high acoustic pressures. MSC adhesion to the phantom vessel wall increased with the time-averaged ultrasound intensity up to 1.65 $W/cm^{2},$ after which further adhesion did not occur. Using higher time-averaged ultrasound intensities may not substantially benefit the adhesion of complexes to the target vessel wall, but could cause undesirable biological effects such as heating to the MB-MSC complexes and surrounding tissue.
Sponsorship	IEEE Ultrasonics, Ferroelectrics Frequency Control Soc.
Starting Page	1886
Ending Page	1889
File Size	1022109
Page Count	4
File Format	PDF
ISBN	9781457712531
ISSN	19485727
e-ISBN	9781457712524
DOI	10.1109/ULTSYM.2011.0471
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-10-18
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ultrasonic imaging Acoustics Force Adhesives USA Councils Phantoms Mathematical model mesenchymal stem cells microbubbles acoustic radiation force intravasvular catheter
Content Type	Text
Resource Type	Article

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