NDLI: Fiber optic fluoro-immunosensor for detection of Salmonella based on evanescent wave absorption

Content Provider	IEEE Xplore Digital Library
Author	Jung, H.J. Yum, S.H. Lee, J.H. Kwon, D.H. Kang, S.W.
Copyright Year	2004
Description	Author affiliation: Dept. of Electron. Eng., Kyungpook Nat. Univ., South Korea (Jung, H.J.)
Abstract	In this study, the fiber optic fluoro-immunosensor was designed to detect Salmonella typhimurium positive control antigen. The fabricated system is consisted of the multimode optical fiber on which goat antiSalmonella was immobilized. And a sandwich assay is applied to the fabricated the fiber optic fluoro-immunosensor. In the 'sandwich' binding format, a primary or 'capture' antibody is immobilized on the core surface of the multimode optical fiber and a secondary or named as 'tracer' antibody was added to the bulk solution. A tracer is that an antibody is labeled Fluorescein isothiocyanate (FITC: /spl lambda//sub ex/=492 nm , /spl lambda//sub em/=520 nm). Concentrations of 0.5, 1, 5, 10, 20, 30, 40 /spl mu/g/ml Salmonella antigen were tested in different fibers. The detection limit of the manufactured system was 1 /spl mu/g/ml, 0.5 /spl mu/g/ml in nonlabeled monolayer phosphate buffered saline (NMP), nonlabeled monolayer carbonate bicarbonate buffer (NMC), respectively.
Sponsorship	IEEE Region 10
Starting Page	668
Ending Page	671
File Size	1967802
Page Count	4
File Format	PDF
ISBN	0780385608
DOI	10.1109/TENCON.2004.1415021
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-11-24
Publisher Place	Thailand
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Absorption Optical fibers Optical surface waves Fluorescence Optical fiber devices Design engineering Optical fiber sensors Optical buffering Optical fiber testing Manufacturing
Content Type	Text
Resource Type	Article

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