NDLI: High-speed three-dimensional fluorescence imaging of neural activity

Content Provider	IEEE Xplore Digital Library
Author	Holy, T.E. Holekam, T.F. Turaga, D.
Copyright Year	2006
Description	Author affiliation: Dept. of Anatomy & Neurobiol., Washington Univ. Sch. of Med., St. Louis, MO (Holy, T.E.; Holekam, T.F.; Turaga, D.)
Abstract	Our progress in understanding the function of the nervous system is, in many ways, limited by our ability to record neural activity. While there has been considerable progress in imaging at the extremes of length scales (e.g., whole brain imaging by fMRI and sub-cellular resolution by two-photon microscopy), there is a need for optical techniques to record the individual activities of large populations of neurons in intact circuits. Current techniques such as epifluorescence microscopy have reasonable time resolution but suffer from signal-to-background problems when viewing thick samples, while confocal techniques are limited in time resolution and/or photon efficiency. To address some of these limitations, we have developed a novel form of microscopy for high-speed three-dimensional fluorescence imaging. Using this technique, entire stacks of high signal-to-noise images can be acquired in seconds. We are currently using this technique to record the activity of populations of sensory neurons in the mouse olfactory system
Starting Page	1
Ending Page	2
File Size	2549498
Page Count	2
File Format	PDF
ISBN	1424402778
DOI	10.1109/LSSA.2006.250367
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-07-13
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence High-resolution imaging Optical imaging Signal resolution Optical microscopy Neurons Nervous system Ultraviolet sources Brain Image resolution
Content Type	Text
Resource Type	Article

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