NDLI: Identification and function of new proteins in calcified endoskeleton: A new insight in the calcification mechanism of soft corals

Content Provider	IEEE Xplore Digital Library
Author	Rahman, M.A. Oomori, T.
Copyright Year	2008
Description	Author affiliation: Dept. of Chem., Univ. of the Ryukyus, Okinawa (Rahman, M.A.; Oomori, T.)
Abstract	Understanding the functional properties of endoskeletal proteins in the calcification processes of soft corals is essential. However, separation of proteins from soft corals is difficult due to contamination by soft tissues and the high sensitivity of soft tissues to handling. We have resolved this problem and established a simple and effective method-electroelution treatment-for purifying proteins from soft corals. Here we applied this newly developed technique to successfully identify four proteins (MPL-1, MPL-2, MPL-3 and MPL-4) from the endoskeletal sclerites of soft coral, Lobophytum crassum as a model. Following this method, we identified a carbonic anhydrase (CA) domain in a soft coral; CA is a key enzyme in living organisms. We found that two CA proteins, which were purified by electroelution, could control the morphology of the $CaCO_{3}$ crystals, and one of these is potentially involved in the process of biocalcification. We report here a single protein (MPL-2), which has both calcium-binding and CA activities and is responsible for $CaCO_{3}$ nucleation and crystal growth. The sequence of protein MPL-2 was subjected to bioinformatics analysis involving identification of similarities to other animals' protein. The function of proteins during bio-calcification was also studied by simulating the nucleation and growth of calcium carbonates in vitro. After precipitation of $CaCO_{3}$ in the experimental design, the obtained crystals were characterized by X-Ray Diffraction (XRD). These findings suggest that the proteins, which were purified from the calcified sclerites, can control the morphology of $CaCO_{3}$ crystals and are potentially involved in the process of bio-calcification.
Starting Page	1
Ending Page	7
File Size	1012650
Page Count	7
File Format	PDF
ISBN	9781424426195
DOI	10.1109/OCEANS.2008.5152121
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-09-15
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Calcium Crystals Biological tissues Morphology Contamination Protein engineering Biochemistry Organisms Bioinformatics Animals
Content Type	Text
Resource Type	Article

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