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Content Provider | IEEE Xplore Digital Library |
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Author | Matuszewski, B.J. Murphy, M.F. Burton, D.R. Marchant, T.E. Moore, C.J. Histace, A. Precioso, F. |
Copyright Year | 2011 |
Description | Author affiliation: Applied Digital Signal and Image Processing Research Centre, UCLan, Preston, UK (Matuszewski, B.J.) || General Engineering Research Institute, Liverpool John Moores University, Liverpool, UK (Murphy, M.F.; Burton, D.R.) || North Western Medical Physics, The Christie NHS Foundation Trust, Manchester, UK (Marchant, T.E.; Moore, C.J.) || ETIS UMR CNRS 8051 (Histace, A.; Precioso, F.) |
Abstract | The paper reports on a novel method for reconstruction of cellular features including cell nuclei and cellular boundaries from actin tagged fluorescence confocal microscopy images. Such reconstruction can provide spatial context for subsequent quantitative analysis of changes to actin organisation and cell morphology in both controlled and stressed cell cultures. The proposed method is fully automatic and is formulated within active contour multiphase level set framework. The derived level set evolution PDEs combine previously proposed curvature and advection flows with propagation flow defined by specially designed set of geodesic distance maps. Additionally the proposed PDEs include additional components to impose known inclusion/exclusion topological constraints between cellular structures. The paper gives an overview of the proposed methodology as well as reports on initial results obtained for monolayer of human prostate cells (PNT2) culture visualised using acting tagged fluorescence confocal microscopy. |
Starting Page | 3081 |
Ending Page | 3084 |
File Size | 1199943 |
Page Count | 4 |
File Format | |
ISBN | 9781457713040 |
ISSN | 15224880 |
e-ISBN | 9781457713033 |
e-ISBN | 9781457713026 |
DOI | 10.1109/ICIP.2011.6116316 |
Language | English |
Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Publisher Date | 2011-09-11 |
Publisher Place | Belgium |
Access Restriction | Subscribed |
Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
Subject Keyword | Image segmentation Level set Microscopy Active contours Fluorescence Mathematical model Equations topological constraints cell segmentation active contour geodesic distance confocal microscopy |
Content Type | Text |
Resource Type | Article |
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