NDLI: Reconstruction of motile actin networks in giant liposome

Content Provider	IEEE Xplore Digital Library
Author	Takiguchi, K. Negishi, M. Tanaka-Takiguchi, Y. Homma, M. Yoshikawa, K.
Copyright Year	2010
Description	Author affiliation: Department of Physics, Graduate School of Science, Kyoto University, Sakyo-ku, 606-8502, Japan (Negishi, M.; Yoshikawa, K.) \|\| Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, 464-8602, Japan (Takiguchi, K.; Tanaka-Takiguchi, Y.; Homma, M.)
Abstract	To construct a simple model cellular system exhibiting the property of self-propelled motion, cell-sized giant liposomes encapsulating desired amounts of actoHMM, a mixture of actin filament (F-actin) and heavy meromyosin (HMM, an actin-related molecular motor), have been prepared. We adapted the methodology of spontaneous transfer of a water droplet through oil/water interface in the presence of phospholipid and successful obtained stable giant liposome with the inner physiological biopolymer solution. We introduced ATP to the bathing solution of liposome encapsulating actoHMM, in which bilayer membrane α-hemolysin, a bacterial membrane pore-forming toxin, is embedded. In this system, ATP is supplied into the inner volume of liposome through the protein pores in a passive manner. Accompanied by the ATP supply, actin networks or bundles that have encapsulated in the liposomes exhibited specific morphological change, being attributable to the active sliding between F-actin and HMM. Remarkable difference in the behavior of F-actins is found; i.e., inside the liposome, almost all the F-actins situate around the inner periphery of the liposome, whereas, in the bulk solution, actin bundles form an aster-like structure.
Starting Page	150
Ending Page	155
File Size	904587
Page Count	6
File Format	PDF
ISBN	9781424479955
e-ISBN	9781424479979
DOI	10.1109/MHS.2010.5669559
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-11-07
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Hidden Markov models Biomembranes Power capacitors Proteins Water Propulsion
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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