NDLI: Biodegradable nanoparticles of alginate and chitosan as non-viral DNA oral delivery system

Content Provider	IEEE Xplore Digital Library
Author	Goncalves, L.M.D. Cadete, A. Figueiredo, L. Calado, C.C.R. Almeida, A.J.
Copyright Year	2011
Description	Author affiliation: Faculdade de Farmácia, Universidade de Lisboa iMed.UL Lisboa, Portugal (Goncalves, L.M.D.; Cadete, A.; Figueiredo, L.; Almeida, A.J.) \|\| Faculdade de Engenharia da Universidade Católica Portuguesa, Rio de Mouro, Portugal.(PI) (Calado, C.C.R.)
Abstract	The delivery of nucleic acids via the oral route involves overcoming barriers such as degradation of nucleic acids by low pH in the stomach, enzymatic degradation by DNases in the gut, crossing the physical barrier imposed by the mucus layer, cellular uptake, intracellular trafficking and nuclear uptake. As an oral drug carrier system chitosan nanoparticles are ideal, being mucoadhesive, interacting with the anionic sialic acid residues in mucin. In this study, plasmid DNA expressing a “humanized” secreted Gaussia Luciferase as reporter gene was encapsulated in alginate and chitosan nanoparticles, via a mild ionotropic gelation procedure with sodium tripolyphosphate as a counterion. The nanoparticle system here developed shows effective transfection of different human gastric epithelial cell lines with distinct cell differention. That was confirmed by the expression of luciferase in the different tested conditions, particularly the amount of encapsulated pGLuc.
Starting Page	1
Ending Page	4
File Size	1212961
Page Count	4
File Format	PDF
ISBN	9781457705229
e-ISBN	9781457705236
e-ISBN	9781457705212
DOI	10.1109/ENBENG.2011.6026051
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-03-01
Publisher Place	Portugal
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Nanoparticles Degradation Proteins Plasmid Tranfection DNA Stability analysis Vaccines Chitosan Cell Immune system
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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