NDLI: Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells

Content Provider	IEEE Xplore Digital Library
Author	Prazeres, D.M.F. Martins, S.A.M. Trabuco, J.R.C. Monteiro, G.A. Juskowiak, A. Conde, J.P. Chu, V.
Copyright Year	2011
Description	Author affiliation: IBB-Institute for Biotechnology and Bioengineering, Lisbon, Portugal (Prazeres, D.M.F.; Martins, S.A.M.; Trabuco, J.R.C.; Monteiro, G.A.; Juskowiak, A.) \|\| Department of Bioengineering, Instituto Superior Técnico, Lisbon, Portugal (Conde, J.P.) \|\| INESC Microsistemas e Nanotechnologias and IN-Institute of Nanoscience and Nanotechnology, Lisbon, Portugal (Chu, V.)
Abstract	G-protein-coupled receptors (GPCRs) play a key role in physiologic and pathologic processes via signal transduction pathways that convert extracellular information into intracellular functions. The identification of drugs that target GPCRs usually relies on functional, cell-based assays. Here, the binding of a test compound to a receptor alters the conformation of the associated G-protein, initiating a cascade of events. The magnitude of the response is typically measured by monitoring key elements in the cascade (e.g., calcium) with fluorescent dyes or with luminescent reporter proteins (e.g., aequorin). The goal of this project is to develop cell microchips for the screening of drugs that act upon GPCRs. The basic unit of the chip will be composed of: i) 15–100 micron-sized test sites where the cells are placed and contacted with the candidate drug via a microfluidic network, and ii) independent, micron-sized amorphous silicon photodetectors that convert the photons of generated light into an electric current and that can be arrayed.
Starting Page	1
Ending Page	4
File Size	379795
Page Count	4
File Format	PDF
ISBN	9781457705229
e-ISBN	9781457705236
e-ISBN	9781457705212
DOI	10.1109/ENBENG.2011.6026042
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-03-01
Publisher Place	Portugal
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs Microchips Calcium G-protein coupled receptors Fluorescence Amorphous silicon Photodetectors Drug discovery Highthroughput Monitoring Microfluidics
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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