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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Guler, Z. Sarac, A.S. |
| Copyright Year | 2015 |
| Description | Author affiliation: Nanosci. & Nanoengineering, Istanbul Tech. Univ., Istanbul, Turkey (Guler, Z.; Sarac, A.S.) |
| Abstract | Poly (ε-caprolactone)/poly (m-anthranilic acid) (PCL/P3ANA) nanofibers were fabricated by electrospinning. Nanofibers were biofuctionalized with Bone morphogenic protein-2 (BMP-2) with 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) surface activation of carboxyl groups (-COOH) on PCL/P3ANA scaffold. The structure of nanofibers was investigated by FTIR-ATR spectroscopy. The amount of bound protein was determined by BCA protein assay. In vitro cell culture studies were conducted by using human osteocarsinoma cell line (SAOS-2) and protein immobilized PCL/P3ANA nanofibers as tissue engineering scaffolds. Cell viability and proliferation assays were performed in order to understand the cell behaviour against PCL/P3ANA nanofibers. Fluorescence staining of F-actin was performed to observe the cell morphology. Alkaline phoshotase (ALP) activity of SAOS-2 cells were investigated. The results confirmed the successful immobilization of BMP-2 on the PCL/P3ANA nanofibers which can be used in bone tissue engineering. |
| Starting Page | 1 |
| Ending Page | 4 |
| File Size | 808979 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781467375443 |
| e-ISBN | 9781467375450 |
| DOI | 10.1109/EHB.2015.7391452 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2015-11-19 |
| Publisher Place | Romania |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Proteins Protein engineering Surface morphology Morphology Fluorescence Poly(antranilic acid) Bones Nanofiber Scaffold Polymers Covalent immobilization |
| Content Type | Text |
| Resource Type | Article |
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