NDLI: On the dynamics of the electric field induced breakdown in lipid membranes

Content Provider	IEEE Xplore Digital Library
Author	Winterhalter, M. Klotz, K.-H. Benz, R.
Copyright Year	1994
Description	Author affiliation: Lehrstuhl fur Biotechnol., Wurzburg Univ., Germany (Winterhalter, M.; Klotz, K.-H.; Benz, R.)
Abstract	Electric fields deliver a unique tool for cell characterization or manipulation. Important biotechnological applications are e.g. electrofusion of cells or electroinjection of macromolecules into living cells. Despite the widespread use little is known about the underlying processes. From a better understanding higher efficiencies are expected. To limit the number of parameters the authors perform a study on planar lipid bilayers. Optical black lipid membranes were formed in the usual way. Rupture was induced by a careful application of short electric field pulses. The measurement of the subsequent increase in membrane conductivity with time allowed conclusions to be made on the underlying mechanism. The initial process of pore formation starts statistically distributed a few /spl mu/sec after the onset of the pulse. The following rupture of the entire lipid membrane is a fast process with an opening velocity of a few cm/s. Decreasing the surface tension of the lipid film by adding surfactant molecules slowed down the rupture. Adsorption of macromolecules decreased the opening velocity. A quantitative measurement was possible by inserting specific amounts of lipids with covalently bound polymers.<>
Starting Page	1462
Ending Page	1469
File Size	570258
Page Count	8
File Format	PDF
ISBN	0780319931
DOI	10.1109/IAS.1994.377618
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1994-10-02
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biomedical optical imaging Electric breakdown Conductivity measurement Lipidomics Biomembranes Time measurement Surface tension Optical films Polymers Optical pulses
Content Type	Text
Resource Type	Article

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