NDLI: Isolation methods of human and bovine adipose tissue derived stem cells

Content Provider	IEEE Xplore Digital Library
Author	Buang, F. Shahimin, M.M.
Copyright Year	2010
Description	Author affiliation: School of Bioprocess Engineering, UniMAP, Malaysia (Buang, F.) \|\| School of Microelectronic Engineering, UniMAP, Malaysia (Shahimin, M.M.)
Abstract	Mammalian adipose tissue derived stem cells have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1 hour and 2 hours under 37°C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1 hours under 37°C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 hours under 37°C in CO2 incubator.
Starting Page	176
Ending Page	179
File Size	1009767
Page Count	4
File Format	PDF
ISBN	9781424475995
e-ISBN	9781424476008
DOI	10.1109/IECBES.2010.5742223
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-11-30
Publisher Place	Malaysia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Human Bovine Tissue engineering Abdominal subcutaneous adipose tissue Humans Medical treatment Stem cells Bones Stem cell isolation
Content Type	Text
Resource Type	Article

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