NDLI: Optical recording of single cardiomyocyte transmembrane potential in Langendorff-perfused mouse hearts

Content Provider	IEEE Xplore Digital Library
Author	Bu, G. Berbari, E.J. Rubart, M.
Copyright Year	2007
Description	Author affiliation: Purdue Univ., West Lafayette, IN (Bu, G.) \|\| Indiana Univ., Indiananpolis, IN (Berbari, E.J.; Rubart, M.)
Abstract	Spatial heterogeneity of action potential properties has been related to cardiac arrhythmogenesis. In this study we used laser scanning confocal microscopy in conjunction with the fast potentiometric dye ANNINE-6 to monitor changes in cardiomyocyte transmembrane potentials in Langendorff-perfused mouse hearts on a subcellular scale. Line-scan images from up to three neighboring cardiomyocytes were obtained during continuous electrical stimulation at 3 Hz. Fluorescence changes for each cardiomyocyte along the scan line were resolved from the corresponding line-scan image. Peak changes in fluorescence intensity during an action potential exceeded 20%. Signal-to-noise ratio of the optical signal was >20. Action potential durations were not significantly different between adjacent cardiomyocytes under our conditions. We conclude that this imaging technique can be used to investigate cell-to-cell repolarization heterogeneity in the intact heart.
Starting Page	357
Ending Page	360
File Size	2493092
Page Count	4
File Format	PDF
ISBN	9781424425334
ISSN	02766547
DOI	10.1109/CIC.2007.4745495
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-09-30
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Computers in Cardiology(CinC)
Subject Keyword	Optical recording Cardiology Mice Heart Fluorescence Laser transitions Microscopy Electrical stimulation Image resolution Signal resolution
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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