Please wait, while we are loading the content...
| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Chen, W. Lee, R.C. |
| Copyright Year | 1994 |
| Description | Author affiliation: Electr. Trauma Res. Program, Chicago Univ., IL, USA (Chen, W.; Lee, R.C.) |
| Abstract | Summary form only given as follows: In addition to electroporation of cell membranes, the authors have also shown shock pulse-induced reduction in voltage-gated ionic channel conductance and channel selectivity, which suggests possible electroconformational changes in proteins of the cell membrane. Therefore, the authors are now attempting to locate the damage in the protein structure. Some of their evidence suggests that the most vulnerable subgroups of channel proteins are located at the narrowest pore of the channels. Experiments were performed using an improved double vaseline-gap voltage and current clamp technique with cut fibers from skeletal muscle of the frog Rana temporaria. The experimental results showed that: 1) the binding of the neurotoxins TIX and TEA on sodium (Na) and potassium (K) channels are not affected by the high voltage electrical shock pulses; 2) the inactivity curve of the delayed rectifier K channel shows little change following electric shock; and 3) K channel conductance and ionic selectivity can be significantly reduced by the electric shock pulse, predicting a depolarization of the membrane resting potential, which has been further proved by directly measuring membrane potential response to an electrical shock. The figure below shows the shock pulse-induced depolarization of the membrane resting potential. Combining these functional alterations of channel proteins, it is possible to answer the question of what subgroups of the channel proteins are the most sensitive to a high voltage electrical shock. The neurotoxin binding sites of TTX and TEA are most likely located on the peripheral residue of the channel proteins not in the narrowest pore of the selectivity filter. Similarly, the well-accepted hypothesis of the channel inactivity suggests that channel inactivity function be correlated to the amino-terminal residues, which are intracellularly located away from the selectivity filter. In contrast, reduction of channel conductance and ionic selectivity are primarily correlated to the narrowest pore of the channels, the selectivity filter. In other words, the narrowest pore of the ion channel with a diameter of a few /spl Aring/ is the most sensitive subgroup to the external electrical field.< |
| File Size | 89316 |
| File Format | |
| ISBN | 0780320506 |
| DOI | 10.1109/IEMBS.1994.412151 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 1994-11-03 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Voltage Electric shock Proteins Biomembranes Pulse measurements Filters Cells (biology) Clamps Muscles Delay |
| Content Type | Text |
| Resource Type | Article |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|