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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Suri, C.R. Mishra, G.C. |
| Copyright Year | 1995 |
| Description | Author affiliation: Inst. of Microbial Technol., Chandigarh, India (Suri, C.R.; Mishra, G.C.) |
| Abstract | We have developed a microgravimetric immunobiosensor for the determination of insulin concentration. The system consisted of a modified piezoelectric quartz crystal having bound to its electrode surface an antibody specific to insulin molecules. The antibody to insulin was covalently immobilized on the modified crystal surface via a direct protein A-gold immobilization method using dimethyl pimelimidate (DMP) as the cross linker. The antibody immobilized via the avidin-biotin immobilization method showed low sensitivity and poor stability of the immune biosensor system. Tests of the activity of the resulting immobilized antibody, uniformly oriented using the protein A-gold immobilization method, indicated that surface density close to the theoretical maximum (close packed) was achieved. A comparison between the present study and the well established radioimmunoassay (RIA) revealed that the described microgravimetric immunoassay technique could be developed as a potential alternate to RIA. |
| File Size | 263667 |
| File Format | |
| ISBN | 078032711X |
| DOI | 10.1109/RCEMBS.1995.533046 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 1995-02-15 |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Monitoring Insulin Immune system Proteins Resonant frequency Surface treatment Molecular biophysics Crystals Chemicals Electrodes |
| Content Type | Text |
| Resource Type | Article |
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