NDLI: Measurement and analysis of defocused point spread functions and optical transfer functions of a microscope

Content Provider	IEEE Xplore Digital Library
Author	Li, J. Agathoklis, P. Peet, F. Jensen, G. Sahota, T.
Copyright Year	1995
Description	Author affiliation: Dept. of Electr. & Comput. Eng., Victoria Univ., BC, Canada (Li, J.; Agathoklis, P.)
Abstract	The defocused point spread functions and optical transfer functions of a microscope were measured and analyzed. The main purpose of the analysis was to verify the accuracy of analytical approximations of these functions, which were presented but not extensively studied in the literature. The measured results were compared with those calculated by Castleman's (1979) theoretical approximation, in the space and frequency domains, graphically and numerically. The results showed that for the numerical apertures used, the theoretical functions were close to the measured functions, and thus Castleman's theoretical approximation is a fairly good approximation. These defocused point spread functions are being used for the optical sectioning as part of microscope image visualization system described by Li, Agathokils, Peet, Jensen and Sahota (see Proceedings of 1993 Canadian Conference on Electrical and Computer Engineering, p.1013-1016, Sept. 1993, Vancouver).
Starting Page	407
Ending Page	410
File Size	422054
Page Count	4
File Format	PDF
ISBN	0780325532
DOI	10.1109/PACRIM.1995.519555
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1995-05-17
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Transfer functions Microwave integrated circuits Biomedical optical imaging Light sources Optical microscopy Optical recording Data visualization Data acquisition Optical computing Forestry
Content Type	Text
Resource Type	Article

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