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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Aguet, F. Van De Ville, D. Unser, M. |
| Copyright Year | 2007 |
| Description | Author affiliation: Biomed. Imaging Group, Ecole Polytech. Fed. de Lausanne (Aguet, F.; Van De Ville, D.; Unser, M.) |
| Abstract | Several recent studies have shown that fluorescent particles can be localized with an accuracy that is well beyond traditional resolution limits. Using a theoretical model of the image formation process that accounts for possible sources of noise, Cramer-Rao bounds have been used to define the theoretical limits. A crucial influence on these bounds is the mismatch of refractive indices that is usually present between immersion medium and specimen. This results in an axially shift-variant point spread function, meaning that the bounds change as a function of the particle's position in the z-direction. We investigate the theoretical bounds for this shift-variant model, and propose a maximum-likelihood estimator for the particle position in 3D (XYZ position). Using this estimator, sub-resolution localization at the nanometer scale is demonstrated on experimental data. The results provide optimal conditions for particle tracking and localization experiments. |
| Starting Page | 932 |
| Ending Page | 935 |
| File Size | 146238 |
| Page Count | 4 |
| File Format | |
| ISBN | 1424406714 |
| DOI | 10.1109/ISBI.2007.357006 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2007-04-12 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Fluorescence Nanoparticles Optical refraction Optical microscopy Optical variables control Maximum likelihood estimation Image resolution Biomedical optical imaging Spatial resolution Optical noise |
| Content Type | Text |
| Resource Type | Article |
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