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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Forster, B. Van De Ville, D. Berent, J. Sage, D. Unser, M. |
| Copyright Year | 2004 |
| Description | Author affiliation: Unser, Swiss Fed. Inst. of Technol., Lausanne, Switzerland (Forster, B.; Van De Ville, D.; Berent, J.; Sage, D.; Unser, M.) |
| Abstract | Microscopy imaging often suffers from limited depth-of-focus. However, the specimen can be 'optically sectioned' by moving the object along the optical axis; different areas appear in focus in different images. Extended depth-of-focus is a fusion algorithm that combines those images into one single sharp composite. One promising method is based on the wavelet transform. In this paper, we show how the wavelet-based image fusion technique can be improved and easily extended to multi-channel data. First, we propose the use of complex-valued wavelet bases, which seem to outperform traditional real-valued wavelet transforms. Second, we introduce a way to apply this technique for multi-channel images that suppresses artifacts and does not introduce false colors, an important requirement for multi-channel fluorescence microscopy imaging. We evaluate our method on simulated image stacks and give results relevant to biological imaging. |
| Starting Page | 660 |
| Ending Page | 663 |
| File Size | 595234 |
| Page Count | 4 |
| File Format | |
| ISBN | 0780383885 |
| DOI | 10.1109/ISBI.2004.1398624 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2004-04-18 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Microscopy Focusing Wavelet transforms Optical imaging Biomedical optical imaging Image fusion Discrete wavelet transforms Frequency Apertures Image reconstruction |
| Content Type | Text |
| Resource Type | Article |
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