NDLI: Development of a Cell-Chip Array for Single Cell Capturing Using Dielectrophoresis

Content Provider	IEEE Xplore Digital Library
Author	Shah, P. Xuena Zhu Chenzhong Li
Copyright Year	2013
Description	Author affiliation: Nanobioengineering/Bioelectronics Lab., Florida Int. Univ., Miami, FL, USA (Shah, P.; Xuena Zhu; Chenzhong Li)
Abstract	Traditional methods of analyzing cells are based on the averaging of results studied from multiple cells in an assay. The results are correlated and assumed to be the equally contributed by all cells of the population under study. However, recent advancement in single cell studies have showed that individual cells behaves differently in the same environment and to study the real functionality of cell, it is really important to study single cells independently. Studying multiple cell assays may obscure some important information which can only be understood by single cell studies. We fabricated a lab on chip device using lithography techniques to study high throughput single cell capturing and analysis. The chip includes microwells on top of active sensing electrode. These electrodes are independently addressable for capturing a single cell in an individual microwell as well as study an independent cell on order. Positive-dielectrophoresis (pDEP) was employed to achieve quick and efficient single cells capturing in individual wells. We examined change in impedance properties of the electrode to verify cell capturing in microwells. Electrochemical characterization of the chip showed reliable behavior.
Sponsorship	Eng. Med. Biol. Soc.
Starting Page	59
Ending Page	60
File Size	617102
Page Count	2
File Format	PDF
ISBN	9781479906246
e-ISBN	9780769550329
DOI	10.1109/SBEC.2013.38
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-05-03
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Impedance Gold Sociology Statistics Spectroscopy Semiconductor device measurement
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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