NDLI: Expression of human fibrinogen in transgenic mice

Content Provider	IEEE Xplore Digital Library
Author	Subramanian, A. Butler, S.P. Gwazdauskas, F.C. Lubon, H. Velander, W.H.
Copyright Year	1997
Description	Author affiliation: Dept. of Chem. Eng., Virginia Polytech. Inst. & State Univ., Blacksburg, VA, USA (Subramanian, A.)
Abstract	Human fibrinogen (hfib) is a 340 kDa plasma protein which is a complex precursor substrate to fibrin during clot formation. The present work evaluates the potential of the mammary gland of transgenic mice to coordinately express three separate cDNAs for hfib and then assemble hexameric, functional recombinant human fibrinogen (rhfib). Trigenic mice were made by pronuclear co-microinjection of separate constructs for each cDNA of the A/spl alpha/, B/spl beta/ and /spl gamma/ hfib polypeptides. Each transgene contained a 2.6 kb promoter sequence from the murine whey acidic protein (mWAP). Trigenic mice expressing rhfib at about 5-35 /spl mu/g/ml milk were generated. The rhfib showed an apparent molecular weight by SDS-PAGE similar to that of hfib under non-reducing conditions. Under reducing conditions, the /spl alpha/-chain of rhfib had slightly greater mobility than /spl alpha/-chain from hfib. The rhfib was converted to fibrin by thrombin in a manner similar to that of hfib where a normal fibrin clot and a lower apparent mobility of the /spl alpha/-chain from the clot relative to the starting precursor chains were observed for both rhfib and hfib. In summary, these results show that functional fibrinogen can be synthesized and secreted by the mammary gland of transgenic mice.
Starting Page	57
Ending Page	60
File Size	353323
Page Count	4
File Format	PDF
ISBN	0780338693
ISSN	10864105
DOI	10.1109/SBEC.1997.583213
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	1997-04-04
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Humans Mice Mammary glands Bioreactors Protein engineering Assembly Animals Plasma chemistry Nuclear and plasma sciences Trigeneration
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression of biologically active human clotting factor IX (hF IX) in the mammary gland of transgenic mice

Comparison of human coagulation factor VIII expression directed by cytomegalovirus and mammary gland-specific promoters in HC11 cells and transgenic mice

Secretion of Human Protein C in Mouse Milk

Expression of human erythropoietin directed by mWAP promoter in mammary gland of transgenic mice

Targeted c-myc gene expression in mammary glands of transgenic mice induces mammary tumours with constitutive milk protein gene transcription.

Transgenic mice carrying an imbalance in the native ratio of A to B forms of progesterone receptor exhibit developmental abnormalities in mammary glands.

Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.

Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.

Gene expression profile and cancer-associated pathways linked to progesterone receptor isoform a (PRA) predominance in transgenic mouse mammary glands

Expression of human fibrinogen in transgenic mice

Similar Documents

Expression of biologically active human clotting factor IX (hF IX) in the mammary gland of transgenic mice

Comparison of human coagulation factor VIII expression directed by cytomegalovirus and mammary gland-specific promoters in HC11 cells and transgenic mice

Secretion of Human Protein C in Mouse Milk

Expression of human erythropoietin directed by mWAP promoter in mammary gland of transgenic mice

Targeted c-myc gene expression in mammary glands of transgenic mice induces mammary tumours with constitutive milk protein gene transcription.

Transgenic mice carrying an imbalance in the native ratio of A to B forms of progesterone receptor exhibit developmental abnormalities in mammary glands.

Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus.

Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin.

Gene expression profile and cancer-associated pathways linked to progesterone receptor isoform a (PRA) predominance in transgenic mouse mammary glands

Expression of human fibrinogen in transgenic mice