NDLI: Genetic Algorithm for the Design of Confronting Two-Pair Primers

Content Provider	IEEE Xplore Digital Library
Author	Cheng-Hong Yang Yu-Huei Cheng Li-Yeh Chuang Hsueh-Wei Chang
Copyright Year	2009
Abstract	Many single nucleotide polymorphisms (SNPs) genotyping techniques have been developed but most of them are expensive. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is a restriction enzyme-free and economic genotyping but its primer design is still computationally challenged. Here, we introduced a genetic algorithm (GA)-based PCR-CTPP primer design method. Thirty SNPs of the Janus kinase 2 gene with their SNP flanking length for 500 bps were tested. These GA-based designing CTPP primers were characterized with close values for melting temperature (Tm) and specificity, and their corresponding PCR products were provided with the optimal length. In conclusion, this novel PCR-CTPP primer designing method provides the computation for the primer information of cost- and time-effective enzyme-free SNP genotyping.
Starting Page	242
Ending Page	247
File Size	252931
Page Count	6
File Format	PDF
ISBN	9780769536569
DOI	10.1109/BIBE.2009.25
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-06-22
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Genetic algorithms Algorithm design and analysis Sequences DNA Biomedical engineering Temperature Biochemistry Engineering in medicine and biology Genetic engineering Design engineering enzyme-free SNP genotyping Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) genetic algorithm (GA)
Content Type	Text
Resource Type	Article

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