NDLI: Photothermal imaging of nanoparticles and cells

Content Provider	IEEE Xplore Digital Library
Author	Zharov, V.P. Lapotko, D.O.
Copyright Year	1995
Abstract	This review summarizes the findings of recent applications of time-domain far-field photothermal (PT) technique to the detection and imaging of nanoscale absorbing particles. This two-beam (pump-probe) technique is based on time-resolved PT visualization of laser-induced thermal effects around nanoparticles. Imaging is accomplished, after an adjustable time delay after the pump laser pulse, with a second probe beam that senses the nanotarget. Using a tunable optical parametric oscillator laser (wavelength, 420 to 570 nm; energy, 0.1-300 /spl mu/J; pulse width, 8 ns) as the pump laser and a Raman shifter (639 nm, 10 nJ, 13 ns) as the probe laser, with a tunable delay of 0 to 5 000 ns of the probe pulse relative to the pump pulse, this approach has demonstrated the capability to visualize nanoscale gold particles (2 to 250 nm) alone and in cells, liposomes (30 to 90 nm), neutral red-stained particles (30 to 500 nm), and polystyrene beads. Different applications of the time-resolved PT technique are discussed, including imaging of absorbing cellular nanostructures and optimization of selective killing of cancer cells and bacteria.
Sponsorship	IEEE Lasers and Electro-Optics Society
Starting Page	733
Ending Page	751
Page Count	19
File Size	852235
File Format	PDF
ISSN	1077260X
Volume Number	11
Issue Number	4
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2005-07-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Nanoparticles Laser excitation Pump lasers Optical imaging Optical pulses Probes Space vector pulse width modulation Visualization Tunable circuits and devices Time domain analysis photothermal (PT) effect Beads cells imaging laser medicine liposomes nanoparticles
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Electrical and Electronic Engineering

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