NDLI: Lensless Miniature Portable Fluorometer for Measurement of Chlorophyll and CDOM in Water Using Fluorescence Contact Imaging

Content Provider	IEEE Xplore Digital Library
Author	Blockstein, L. Yadid-Pecht, O.
Copyright Year	2009
Abstract	We report on the design, fabrication, and verification of a proof-of-concept miniature fluorometer, which is designed to measure chlorophyll and colored dissolved organic matter (CDOM) concentration in aquatic environment. The system utilizes light emitting diodes (LEDs) for fluorescence excitation and absorption filters for excitation light attenuation. The excitation LED for chlorophyll has peak emission at 465 nm, and the excitation LED for CDOM has peak emission at 341 nm. Our device demonstrates the concept of attaching two different absorption filters on a single sensor array for measuring the fluorescence signal from two different fluorescent dyes. We have tested the system's ability to detect fluorescence from various concentrations of fluorescein as a close simulant and the calibration standard for chlorophyll, and quinine sulfate dihydrate (QSD) as a simulant and calibration standard for CDOM. We have successfully acquired the fluorescent signal for fluorescein between 0.7 and 1000 nM and for QSD between 2.6 and 638 nM.
Starting Page	1
Ending Page	16
Page Count	16
File Size	1178142
File Format	PDF
ISSN	19430655
Volume Number	6
Issue Number	3
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-01-01
Publisher Place	U.S.A.
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Optical filters Fluorescence Water resources Absorption Sea surface Arrays Monitoring fluorescent contact imaging Contact imaging lensless system lensfree system fluorescence fluorescein quinine sulfate dihydrate CDOM chlorophyll
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Electrical and Electronic Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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