NDLI: Electrode design improvement for impedance evaluation of biological cell culture under variable frequency low intensity sinusoidal electric field

Content Provider	IEEE Xplore Digital Library
Author	Chaudhuri, C.R. Mondal, D.
Copyright Year	1994
Abstract	In this paper the electrode geometry effects on the impedance spectroscopy of biological cell culture have been studied under low intensity sinusoidal electric field with a view to design improved electrode structures for continuous cell growth monitoring. To achieve this, impedance estimation has been done by modified distributed analysis both in presence and absence of confluent cell layer by taking into account the effect of solution resistance and the separation between the electrodes. The model has been applied on fibroblast cell culture (3T3) in the frequency range of 10 Hz to 10 kHz. It has been observed that for most of the frequencies, the resistance sensitivity (S_r) is less than the capacitance sensitivity (S_c) and does not increase monotonically with radius of working electrode (R_e) unlike S_c. Further, the nature of variation of S_r and S_c are fairly contradictory with variation in Re and separation between the electrodes (d-Re). Thus an optimum geometry has been selected based on a figure of merit (FOM) which has been defined as the product of S_r and S_c. The maximum FOM corresponds to R_e=90 μm and d=3R_e for a typical frequency of 4 kHz. Observations at 4 kHz show that the optimized geometry yields values of Sr that are almost 5 times higher than those from the conventional geometry (R_e=125 μm and d=300R_e). This increase permits the small signals resulting from the micromotion of cells to be more easily seen.
Sponsorship	National Natural Science Foundation of China (NSFC) Region Centre Ville de Tours Conseil General D'Indre et Loire ABB IEEE Sandia National Laboratories TOSHIBA
Starting Page	382
Ending Page	390
Page Count	9
File Size	776572
File Format	PDF
ISSN	10709878
Volume Number	20
Issue Number	2
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Impedance Electrodes Mathematical model Equations Immune system Resistance Capacitance optimization Biological cells distributed estimation electrode impedance measurement
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Evaluation of percutaneous electrode after implantation for FES

Design of Return/Ground Electrode for Neural Stimulator

The Rectification Properties of an Electrode-Electrolyte Interface Operated at High Sinusoidal Current Density

Electrode-electrolyte Interface Impedance: The Limit Current Of Linearity

Electrical Properties of Body Tissues and Impedance Plethysmography

Epidermal A.C. impedance: low frequency distortions

Non linear fractal impedance model of an electrode-electrolyte interface

Impedance Imaging Electrodes - A New Measurement Technique

Electrode polarization impedance in weak NaCl aqueous solutions

Electrode design improvement for impedance evaluation of biological cell culture under variable frequency low intensity sinusoidal electric field

Similar Documents

Evaluation of percutaneous electrode after implantation for FES

Design of Return/Ground Electrode for Neural Stimulator

The Rectification Properties of an Electrode-Electrolyte Interface Operated at High Sinusoidal Current Density

Electrode-electrolyte Interface Impedance: The Limit Current Of Linearity

Electrical Properties of Body Tissues and Impedance Plethysmography

Epidermal A.C. impedance: low frequency distortions

Non linear fractal impedance model of an electrode-electrolyte interface

Impedance Imaging Electrodes - A New Measurement Technique

Electrode polarization impedance in weak NaCl aqueous solutions

Electrode design improvement for impedance evaluation of biological cell culture under variable frequency low intensity sinusoidal electric field