NDLI: Microorifice-Based High-Yield Cell Fusion on Microfluidic Chip: Electrofusion of Selected Pairs and Fusant Viability

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Microorifice-Based High-Yield Cell Fusion on Microfluidic Chip: Electrofusion of Selected Pairs and Fusant Viability

Content Provider	IEEE Xplore Digital Library
Author	Gel, M. Suzuki, S. Kimura, Y. Kurosawa, O. Techaumnat, B. Oana, H. Washizu, M.
Copyright Year	2002
Abstract	Microorifice-based fusion makes use of electric field constriction to assure high-yield one-to-one fusion of selected cell pairs. The aim of this paper is to verify feasibility of high-yield cell fusion on a microfluidic chip. This paper also examines viability of the fusant created on the chip. We fabricated a microfluidic chip to fuse selected cell pairs and to study postfusion behavior. We used a self-forming meniscus-based fabrication process to create microorifice with a diameter of 2-10 ¿m on the vertical walls in a microfluidic channel. When 1 MHz was applied to electrodes located on both sides of the microorifice, dielectrophoretic force attracted the cells toward microorifice to form a cell pair. Once the cells get into contact, fusion pulse was applied. Real time imaging of cells during fusion and cytoplasmic dye transfer between cells indicated success of cell fusion. We found that when high frequency voltage for dielectrophoresis was swept from 1 MHz to 10 kHz in 100 ¿s, cell fusion was initiated. The effective electric field strength was 0.1-0.2 kV/cm. We analyzed viability by imaging fusant going into cell division phase after 48 h of incubation. We conclude that fabricated microfluidic chip is suitable for high-yield one-to-one fusion and creation of viable fusants. This technology should be a useful tool to study fusion phenomena and viability of fusants, as it allows imaging of the cells during and after the fusion.
Sponsorship	IEEE Engineering in Medicine and Biology Society IEEE Systems, Man, and Cybernetics Society IEEE Nanotechnology Council IEEE Computational Intelligence Society IEEE Robotics and Automation Society IEEE Computer Society
Page Count	6
File Size	1218433
Starting Page	300
Ending Page	305
File Format	PDF
ISSN	15361241
Volume Number	8
Issue Number	4
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-12-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microfluidics Biomembranes Fusion power generation Dielectrophoresis Educational technology Mechanical engineering Chemical hazards Fuses Fabrication Electrodes micro orifice Cell fusion electrofusion microfabrication microfluidic chip
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Bioengineering Electrical and Electronic Engineering Biomedical Engineering Computer Science Applications Biotechnology Pharmaceutical Science

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