NDLI: Identification of <emphasis emphasistype=italic>Listeria</emphasis> Species Using a Low-Cost Surface-Enhanced Raman Scattering System With Wavelet-Based Signal Processing

Content Provider	IEEE Xplore Digital Library
Author	Green, G.C. Chan, A.D.C. Luo, B.S. Hanhong Dan Min Lin
Copyright Year	1963
Abstract	We investigated the ability to distinguish between six species within the Listeria genus (including the human pathogen Listeria monocytogenes ) based on a bacteria sample's surface-enhanced Raman scattering (SERS) spectrum. Our measurement system consists of a portable low-cost Raman spectral acquisition unit and associated signal processing and classification modules. First, Listeria was cultured and then adsorbed onto silver colloidal nanoparticles for SERS measurements. A total of 483 SERS spectra were collected and preprocessed (using a stationary wavelet transform decomposition) to remove noise and baseline artifact. Distinguishing features were extracted by retaining detail wavelet coefficients of significant value across multiple scales. Using a linear classifier in association with ldquoleave one outrdquo cross-validation, the system achieved maximum classification accuracies of 96.1% (six-category) and 97.9% (two-category, L. monocytogenes versus all others). Dimensionality reduction was used to decrease the number of features from 74 to 5 while maintaining similar classification accuracy. In the future, it is envisioned that a measurement system such as this, which is a combination of low-cost hardware with sophisticated signal processing, could play a complementary role with existing methods in realizing a rapid inexpensive means of identifying food-borne bacterial pathogens.
Sponsorship	IEEE Instrumentation and Measurement Society Bureau international des poids et mesures IEEE International Bureau of Weights and Measures (BIPM) URSI NBS Agency Ind. Sci. & Technol., Min. Int. Trade & Ind. Sci. Council Japan International Union of Pure and Applied Physics (IUPAP) NIST NRCC National Conference of Standards Laboratories (NCSL) Allen Osborne Associates Andeen-Hagerling, Inc. Ballantine Lab. Inc. Clarke-Hess Communications Research Corp. Fluke Corporation Guildline Instruments Hewlett-Packard Co. Julie Res. Lab. Inc. Keithley Instruments, Inc. Measurements International Ltd. Canada QuadTech Inc. Quantum Design Inc. Rotek Instrum. Corp. Tegam Inc. Tektronix Inc
Starting Page	3713
Ending Page	3722
Page Count	10
File Size	1232041
File Format	PDF
ISSN	00189456
Volume Number	58
Issue Number	10
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-10-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Pathogens Microorganisms Signal processing Humans Raman scattering Silver Nanoparticles Wavelet transforms Feature extraction Wavelet coefficients wavelet transforms Biomedical signal processing feature extraction Listeria pattern classification Raman spectroscopy surface-enhanced Raman scattering (SERS)
Content Type	Text
Resource Type	Article
Subject	Instrumentation Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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