NDLI: Protocol development using an electronic nose for differentiating E. coli strains

Content Provider	IEEE Xplore Digital Library
Author	Alocilja, E.C. Ritchie, N.L. Grooms, D.L.
Copyright Year	2001
Abstract	The presence of Escherichia coli (E. coli) O157:H7 in the food industry continues to be a problem in America. The pathogenic bacteria, commonly associated with beef products, are the cause of numerous human health concerns. On the other hand, the nontoxic E. coli is present in the intestinal tracts of animals and humans and helps in digestion. Thus, the long-term research objective is to differentiate quickly the pathogen E. coli O157:H7 from the nontoxic strains of E. coli in the farm-to-fork industry chain before the infected food reaches the consumer. This paper describes a constructed gas-sensor electronic-nose system that detects the released gases (amines and alcohol compounds) occurring during the growth of the bacteria cultured in two types of media, namely nutrient broth and brain heart infusion (BHI) broth. The output is a gas signature for each bacterial sample. Eight different E. coli samples were used in testing; four O157:H7 and four non-O157:H7 isolates. Different concentrations of bacteria were used in the study, ranging from 10/sup 2/ to 10/sup 7/ colony-forming units per milliliter. The results of the research showed that E. coli O157:H7 had a distinctly different gas signature than that of the non-O157:H7 bacteria when grown in nutrient broth. Bacteria grown in BHI broth did not have differentiating results. The initiation of gas increase in the bacteria correlated directly with the initial cell concentration of the sample. The initial increase of gas released by the bacteria occurred during the exponential phase of bacterial growth. The electronic nose device was able to differentiate the gas signatures of non-O157:H7 E. coli and O157:H7 E. coli isolates grown in nutrient broth with excellent reproducibility. Potential applications include food and environmental safety from farm to fork.
Sponsorship	IEEE Sensors Council
Starting Page	801
Ending Page	805
Page Count	5
File Size	356326
File Format	PDF
ISSN	1530437X
Volume Number	3
Issue Number	6
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2003-12-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Protocols Electronic noses Capacitive sensors Microorganisms Food industry Pathogens Humans Intestines Animals Gases
Content Type	Text
Resource Type	Article
Subject	Instrumentation Electrical and Electronic Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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