NDLI: Studies on the Physical Characteristics of the Radio-Frequency Atmospheric-Pressure Glow Discharge Plasmas for the Genome Mutation of Methylosinus trichosporium

Content Provider	IEEE Xplore Digital Library
Author	He-Ping Li Zhi-Bin Wang Nan Ge Pei-Si Le Hao Wu Yuan Lu Li-Yan Wang Chong Zhang Cheng-Yu Bao Xin-Hui Xing
Copyright Year	1973
Abstract	In this paper, both simulations and experiments are conducted to investigate the physical characteristics of the radio-frequency atmospheric-pressure glow discharges (RF APGDs) first. The experimental and modeling results show that there are abundant chemically reactive species, e.g., electrons, helium metastables and ions, with high number densities in the discharge region. The corresponding plasma jet temperatures are in the range of 27^°C - 39^°C with the power input lower than 120 W, which is suitable for the treatment of the biomolecules or cells. Then, a prototype, which is the so-called atmospheric and room-temperature plasma mutation system, is designed with the RF APGD plasma generator as the core component. As an example, it is used for the mutation breeding of Methylosinus trichosporium OB3b. The experimental results show that the RF APGD plasma source is an environmentally friendly and efficient tool for the genome mutation of microorganisms to construct mutant library with large diversity.
Sponsorship	IEEE Nuclear and Plasma Sciences Society
Starting Page	2853
Ending Page	2860
Page Count	8
File Size	941779
File Format	PDF
ISSN	00933813
Volume Number	40
Issue Number	11
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-11-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Radio frequency Helium Temperature measurement Plasma temperature Voltage measurement Genomics radio frequency (RF) Atmospheric-pressure glow discharge biological breeding genome mutation
Content Type	Text
Resource Type	Article
Subject	Nuclear and High Energy Physics Condensed Matter Physics

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