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Inflight microbial monitoring-an alternative method to culture based detection currently used on international space station
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Roman, Monsi Smith, David J. Birmele, Michele N. Khodadad, Christina L. Hummerick, Mary E. Wheeler, Raymond M. |
| Copyright Year | 2015 |
| Description | Previous research has shown that microorganisms and potential human pathogens have been detected on the International Space Station (ISS). The potential to introduce new microorganisms occurs with every exchange of crew or addition of equipment or supplies. Previous research has shown that microorganisms introduced to the ISS are readily transferred between crew and subsystems and back (i.e. ECLSS, environmental control and life support systems). Current microbial characterization methods require enrichment of microorganisms and a 48-hour incubation time. This increases the microbial load while detecting a limited number of microorganisms. The culture based method detects approximately 1-10% of the total organisms present and provides no identification, To identify and enumerate ISS samples requires that samples to be returned to Earth for complete analysis. Therefore, a more expedient, low-cost, in-flight method of microbial detection, identification, and enumeration is warranted. The RAZOR EX, a ruggedized, commercial off the shelf, real-time PCR field instrument was tested for its ability to detect microorganism at low concentrations within one hour. Escherichia coli, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa were detected at low levels using real-time DNA amplification. Total heterotrophic counts could also be detected using a 16S gene marker that can identify up to 98% of all bacteria. To reflect viable cells found in the samples, RNA was also detectable using a modified, single-step reverse transcription reaction. |
| File Size | 441610 |
| Page Count | 9 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20150022485 |
| Archival Resource Key | ark:/13960/t35190w7z |
| Language | English |
| Publisher Date | 2015-07-12 |
| Access Restriction | Open |
| Subject Keyword | Inflight Microbial International Space Station Culture Salmonella Escherichia Microbiology Consumables Spacecrew Supplies Pseudomonas Environmental Control Commercial Off-the-shelf Products Prevention Life Support Systems Microorganisms Ribonucleic Acids Low Cost Deoxyribonucleic Acid In-flight Monitoring Spacecraft Environments Detection Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
