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Inflight microbial monitoring- an alternative method to culture based detection currently used on the international space station
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Roman, Monsi Smith, David J. Birmele, Michele N. Khodadad, Christina L. Hummerick, Mary E. Wheeler, Raymond M. |
| Copyright Year | 2015 |
| Description | Previous research has shown that potentially destructive microorganisms and human pathogens have been detected on the International Space Station (ISS). The likelihood of introducing new microorganisms occurs with every exchange of crew or addition of equipment or supplies. Microorganisms introduced to the ISS are readily transferred between crew and subsystems (i.e. ECLSS, environmental control and life support systems). Current microbial characterization methods require enrichment of microorganisms and at least a 48-hour incubation time. This increases the microbial load while detecting only a limited number of the total microorganisms. The culture based method detects approximately 1-10% of the total organisms present and provides no identification. To identify and enumerate ISS microbes requires that samples be returned to Earth for complete analysis. Therefore, a more expedient, low-cost, in-flight method of microbial detection, identification, and enumeration is warranted. The RAZOR EX, a ruggedized, commercial off the shelf, real-time PCR field instrument was tested for its ability to detect microorganisms at low concentrations within one hour. Escherichia coli, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa were detected at low levels using real-time DNA amplification. Total heterotrophic counts could also be detected using a 16S gene marker that can identify up to 98% of all bacteria. To reflect viable cells found in the samples, RNA was also detectable using a modified, single-step reverse transcription reaction. |
| File Size | 390843 |
| Page Count | 8 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20150014974 |
| Archival Resource Key | ark:/13960/t2r54n52g |
| Language | English |
| Publisher Date | 2015-07-12 |
| Access Restriction | Open |
| Subject Keyword | Inflight Microbial Based Detection Iss Salmonella Escherichia Microbiology Consumables Spacecrew Supplies Pseudomonas Environmental Control Commercial Off-the-shelf Products Prevention Life Support Systems International Space Station Microorganisms Ribonucleic Acids Low Cost Deoxyribonucleic Acid In-flight Monitoring Spacecraft Environments Detection Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
