NDLI: Karyotyping of chromosomes in human bronchial epithelial cells transformed by high energy fe ions

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Karyotyping of chromosomes in human bronchial epithelial cells transformed by high energy fe ions

Content Provider	NASA Technical Reports Server (NTRS)
Author	Zhang, Ye Park, Seongmi Story, Michael D. Wu, Honglu Wilson, Bobby Yeshitla, Samrawit
Copyright Year	2015
Description	Lung cancer induced from exposures to space radiation is one of the most significant health risks for long-term space travels. Evidences show that low- and high- Linear energy transfer (LET)-induced transformation of normal human bronchial epithelial cells (HBEC) that are immortalized through the expression of Cdk4 and hTERT. The cells were exposed to gamma rays and high-energy Fe ions for the selection of transformed clones. Transformed HBEC are identified and analyzed chromosome aberrations (i.e. genomic instability) using the multi-color fluorescent in situ hybridization (mFISH), as well as the multi-banding in situ hybridization (mBAND) techniques. Our results show chromosomal translocations between different chromosomes and several of the breaks occurred in the q-arm of chromosome 3. We also identified copy number variations between the transformed and the parental HBEC regardless of the exposure conditions. We observed chromosomal aberrations in the lowand high-LET radiation-induced transformed clones and they are imperfectly different from clones obtain in spontaneous soft agar growth.
File Size	625802
Page Count	1
File Format	PDF
Alternate Webpage(s)	http://archive.org/details/NASA_NTRS_Archive_20150001879
Archival Resource Key	ark:/13960/t08w8dt5d
Language	English
Publisher Date	2015-01-13
Access Restriction	Open
Subject Keyword	Aerospace Medicine Gamma Rays Mutations Lungs Health Bronchi Risk Exposure Extraterrestrial Radiation Radiation Dosage Chromosome Aberrations Cancer Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science
Content Type	Text
Resource Type	Presentation

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