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Simplified microarray technique for identifying mrna in rare samples
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Almeida, Eduardo Kadambi, Geeta |
| Copyright Year | 2007 |
| Description | Two simplified methods of identifying messenger ribonucleic acid (mRNA), and compact, low-power apparatuses to implement the methods, are at the proof-of-concept stage of development. These methods are related to traditional methods based on hybridization of nucleic acid, but whereas the traditional methods must be practiced in laboratory settings, these methods could be practiced in field settings. Hybridization of nucleic acid is a powerful technique for detection of specific complementary nucleic acid sequences, and is increasingly being used for detection of changes in gene expression in microarrays containing thousands of gene probes. A traditional microarray study entails at least the following six steps: 1. Purification of cellular RNA, 2. Amplification of complementary deoxyribonucleic acid [cDNA] by polymerase chain reaction (PCR), 3. Labeling of cDNA with fluorophores of Cy3 (a green cyanine dye) and Cy5 (a red cyanine dye), 4. Hybridization to a microarray chip, 5. Fluorescence scanning the array(s) with dual excitation wavelengths, and 6. Analysis of the resulting images. This six-step procedure must be performed in a laboratory because it requires bulky equipment. |
| File Size | 294130 |
| Page Count | 2 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_20090040763 |
| Archival Resource Key | ark:/13960/t7rn84h4f |
| Language | English |
| Publisher Date | 2007-01-01 |
| Access Restriction | Open |
| Subject Keyword | Technology Utilization And Surface Transportation Amplification Complementary Dna Ribonucleic Acids Deoxyribonucleic Acid Proving Fluorescence Identifying Excitation Nucleic Acids Gene Expression Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
