Loading...
Please wait, while we are loading the content...
Similar Documents
Nucleotide-protectable labeling of sulfhydryl groups in subunit i of the atphase from halobacterium saccharovorum
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Hochstein, Lawrence I. Sulzner, Michael Stan-Lotter, Helga |
| Copyright Year | 1992 |
| Description | A membrane-bound ATPase from the archaebacterium Halobacterium saccharovorum is inhibited by N-ethyl-maleimide in a nucleotide-protectable manner. When the enzyme was incubated with N-[C-14]jethylmaleimide, the bulk of radioactivity was as- sociated with the 87,000-Da subunit (subunit 1). ATP, ADP, or AMP reduced incorporation of the inhibitor. No charge shift of subunit I was detected following labeling with N-ethylmaleimide, indicating an electroneutral reaction. The results are consistent with the selective modification of sulfhydryl groups in subunit I at or near the catalytic site and are further evidence of a resemblance between this archaebacterial ATPase and the vacuolar-type ATPases. |
| File Size | 332885 |
| Page Count | 4 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19980008069 |
| Archival Resource Key | ark:/13960/t4dn9294c |
| Language | English |
| Publisher Date | 1992-07-01 |
| Access Restriction | Open |
| Subject Keyword | Enzyme Activity Thiols Inhibitors Ethyl Compounds Catalysts Halophiles Bacteria Amino Acids Nucleotides Carbon 14 Adenosine Triphosphate Radioactivity Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
