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Gene-based detection of microorganisms in environmental samples using pcr
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Lefkowitz, Elliot J. Wechser, Mark Cassell, Gail H. Paszko-Kolva, Christine Glass, John I. Albin, Michael Roman, Monsi C. Taylor, Theresa B. |
| Copyright Year | 1997 |
| Description | Contaminating microorganisms pose a serious potential risk to the crew's well being and water system integrity aboard the International Space Station (ISS). We are developing a gene-based microbial monitor that functions by replicating specific segments of DNA as much as 10(exp 12) x. Thus a single molecule of DNA can be replicated to detectable levels, and the kinetics of that molecule's accumulation can be used to determine the original concentration of specific microorganisms in a sample. Referred to as the polymerase chain reaction (PCR), this enzymatic amplification of specific segments of the DNA or RNA from contaminating microbes offers the promise of rapid, sensitive, quantitative detection and identification of bacteria, fungi, viruses, and parasites. We envision a small instrument capable of assaying an ISS water sample for 48 different microbes in a 24 hour period. We will report on both the developments in the chemistry necessary for the PCR assays to detect microbial contaminants in ISS water, and on progress towards the miniaturization and automation of the instrumentation. |
| File Size | 1028272 |
| Page Count | 10 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19970027952 |
| Archival Resource Key | ark:/13960/t7kq2xx84 |
| Language | English |
| Publisher Date | 1997-01-01 |
| Access Restriction | Open |
| Subject Keyword | Enzyme Activity Genes Measuring Instruments Viruses Parasites Contamination Environmental Control Fungi Molecules International Space Station Microorganisms Ribonucleic Acids Deoxyribonucleic Acid Water Quality Automatic Control Spacecraft Environments Miniaturization Sampling Detection Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
