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Heavy ion induced mutations in mammalian cells: cross sections and molecular analysis
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Schneider, E. Schmidt, P. Stoll, U. Kiefer, J. |
| Copyright Year | 1994 |
| Description | Our investigations of heavy ion-induced mutations in mammalian cells, which had been begun a few years ago, were systematically continued. For the first time, it was possible to cover a large LET range with a few kinds of ions. To do this, both UNILAC and SIS were used to yield comparable data for a large energy range. This is a necessary condition for a comprehensive description of the influence of such ion parameters as energy and LET. In these experiments, the induced resistance against the poison 6-thioguanin (6-TG), which is linked to the HPRT locus on the genome, is being used as mutation system. In addition to the mutation-induction cross-section measurements, the molecular changes of the DNA are being investigated by means of Multiplex PCR ('Polymerase Chain Reaction') gene amplification. From these experiments we expect further elucidation of the mutation-inducing mechanisms composing the biological action of heavy-ion radiation. |
| File Size | 134241 |
| Page Count | 3 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19950026307 |
| Archival Resource Key | ark:/13960/t4xh4j14z |
| Language | English |
| Publisher Date | 1994-01-01 |
| Access Restriction | Open |
| Subject Keyword | Multiplexing Radiation Effects Mutations Heavy Ions Molecular Structure Molecular Dynamics Mammals Loci Amplification Ionizing Radiation Ion Irradiation Cells Biology Molecular Physics Linear Energy Transfer Let Deoxyribonucleic Acid Radiation Damage Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
