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Inactivation, dna double strand break induction and their rejoining in bacterial cells irradiated with heavy ions
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Schaefer, M. Zimmermann, H. Schmitz, C. |
| Copyright Year | 1994 |
| Description | Besides inactivation one of the major interests in our experiments is to study the primary damage in the DNA double strand breaks (DSB) after heavy ion irradiation. These damages lead not only to cell death but also under repair activities to mutations. In further experiments we have investigated the inactivation with two different strains of Deinococcus radiodurans (R1, Rec 30) and the induction of DSB as well as the rejoining of DSB in stationary cells of E. coli (strain B/r) irradiated with radiations of different quality. In the latter case irradiations were done so that the cell survival was roughly at the same level. We measured the DSB using the pulse field gelelectrophoresis which allows to separate between intact (circular) and damaged (linear) DNA. The irradiated cells were transferred to NB medium and incubated for different times to allow rejoining. |
| File Size | 142974 |
| Page Count | 5 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19950026303 |
| Archival Resource Key | ark:/13960/t02z6567g |
| Language | English |
| Publisher Date | 1994-01-01 |
| Access Restriction | Open |
| Subject Keyword | Radiation Effects Escherichia Mutations Heavy Ions Deactivation Gels Strands Maintenance Survival Ion Irradiation Cells Biology Deoxyribonucleic Acid Bacteria Death Electrophoresis Molecular Biology Chromosomes Radiation Damage Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
