NDLI: Horizontally rotated cell culture system with a coaxial tubular oxygenator

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Horizontally rotated cell culture system with a coaxial tubular oxygenator

Content Provider	NASA Technical Reports Server (NTRS)
Copyright Year	1991
Description	The present invention relates to a horizontally rotating bioreactor useful for carrying out cell and tissue culture. For processing of mammalian cells, the system is sterilized and fresh fluid medium, microcarrier beads, and cells are admitted to completely fill the cell culture vessel. An oxygen containing gas is admitted to the interior of the permeable membrane which prevents air bubbles from being introduced into the medium. The cylinder is rotated at a low speed within an incubator so that the circular motion of the fluid medium uniformly suspends the microbeads throughout the cylinder during the cell growth period. The unique design of this cell and tissue culture device was initially driven by two requirements imposed by its intended use for feasibility studies for three dimensional culture of living cells and tissues in space by JSC. They were compatible with microgravity and simulation of microgravity in one G. The vessels are designed to approximate the extremely quiescent low shear environment obtainable in space.
File Size	711083
Page Count	7
File Format	PDF
Alternate Webpage(s)	http://archive.org/details/NASA_NTRS_Archive_19910021353
Archival Resource Key	ark:/13960/t4pk59754
Language	English
Publisher Date	1991-06-25
Access Restriction	Open
Subject Keyword	Rotating Fluids Cells Biology Gravitational Effects Bioreactors Space Commercialization Tissues Biology Culture Techniques Microgravity Rotation Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science
Content Type	Text
Resource Type	Patent

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