NDLI: Hormone purification by isoelectric focusing in space (Document No: 19890010911)

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Hormone purification by isoelectric focusing in space (Document No: 19890010911)

Content Provider	NASA Technical Reports Server (NTRS)
Author	Bier, M.
Copyright Year	1988
Description	The objective of the program was the definition and development of optimal methods for electrophoretic separations in microgravity. The approach is based on a triad consisting of ground based experiments, mathematical modeling and experiments in microgravity. Zone electrophoresis is a rate process, where separation is achieved in uniform buffers on the basis of differences in electrophoretic mobilities. Optimization and modeling of continuous flow electrophoresis mainly concern the hydrodynamics of the flow process, including gravity dependent fluid convection due to density gradients and gravity independent electroosmosis. Optimization of focusing requires a more complex model describing the molecular transport processes involved in electrophoresis of interacting systems. Three different focusing instruments were designed, embodying novel principles of fluid stabilization. Fluid stability was achieved by: (1) flow streamlining by means of membrane elements in combination with rapid fluid recycling; (2) apparatus rotation in combination with said membrane elements; and (3) shear stress induced by rapid recycling through a narrow gap channel.
File Size	864621
Page Count	14
File Format	PDF
Alternate Webpage(s)	http://archive.org/details/NASA_NTRS_Archive_19890010911
Archival Resource Key	ark:/13960/t6zw6bq5b
Language	English
Publisher Date	1988-10-01
Access Restriction	Open
Subject Keyword	Materials Processing Proteins Purification Fluid Dynamics Space Commercialization Space Processing Electrophoresis Mathematical Models Hormones Microgravity Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science
Content Type	Text
Resource Type	Technical Report

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