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Heterogeneity in the growth hormone pituitary gland system of rats and humans: implications to microgravity based research
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Hymer, W. C. Cleveland, C. Lanham, J. W. Hayes, C. Todd, P. Morrison, Dennis R. Grindeland, R. |
| Copyright Year | 1988 |
| Description | The cell separation techniques of velocity sedimentation, flow cytometry and continuous flow electrophoresis were used to obtain enriched populations of growth hormone (GH) cells. The goal was to isolate a GH cell subpopulation which releases GH molecules which are very high in biological activity, it was important to use a method which was effective in processing large numbers of cells over a short time span. The techniques based on sedimentation are limited by cell density overlaps and streaming. While flow cytometry is useful in the analytical mode for objectively establishing cell purity, the numbers of cells which can be processed in the sort mode are so small as to make this approach ineffective in terms of the long term goals. It was shown that continuous flow electrophoresis systems (CFES) can separate GH cells from other cell types on the basis of differences in surface charge. The bioreactive producers appear to be more electrophoretically mobile than the low producers. Current ground based CFES efforts are hampered by cell clumping in low ionic strength buffers and poor cell recoveries from the CFES device. |
| File Size | 10950396 |
| Page Count | 42 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19890010908 |
| Archival Resource Key | ark:/13960/t6159gq60 |
| Language | English |
| Publisher Date | 1988-10-01 |
| Access Restriction | Open |
| Subject Keyword | Materials Processing Bioprocessing Pituitary Hormones Purification Rats Heterogeneity Cytometry Human Beings Cells Biology Settling Space Commercialization Space Processing Electrophoresis Pituitary Gland Microgravity Polarization Charge Separation Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Technical Report |
