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Distribution and abundance of organic thiols
| Content Provider | NASA Technical Reports Server (NTRS) |
|---|---|
| Author | Fahey, R. |
| Copyright Year | 1985 |
| Description | The role of glutathione (GSH) in protecting against the toxicity of oxygen and oxygen by products is well established for all eukaryotes studied except Entamoeba histolytica which lacks mitochrondria, chloroplasts, and microtubules. The GSH is not universal among prokaryotes. Entamoeba histolytica does not produce GSH or key enzymes of GSH metabolism. A general method of thiol analysis based upon fluorescent labeling with monobromobimane and HPLC separation of the resulting thiol derivatives was developed to determine the occurrence of GSH and other low molecular weight thiols in bacteria. Glutathione is the major thiol in cyanobacteria and in most bacteria closely related to the purple photosynthetic bacteria, but GSH was not found in archaebacteria, green bacteria, or GRAM positive bacteria. It suggested that glutathione metabolism was incorporated into eukaryotes at the time that mitochondria and chloroplasts were acquired by endosymbiosis. In Gram positive aerobes, coenzyme A occurs at millimolar levels and CoA disulfide reductases are identified. The CoA, rather than glutathione, may function in the oxygen detoxification processes of these organisms. |
| File Size | 79316 |
| Page Count | 1 |
| File Format | |
| Alternate Webpage(s) | http://archive.org/details/NASA_NTRS_Archive_19850024404 |
| Archival Resource Key | ark:/13960/t1fj7bj8f |
| Language | English |
| Publisher Date | 1985-07-01 |
| Access Restriction | Open |
| Subject Keyword | Oxygen Thiols Archaebacteria Toxicity Prokaryotes Aerobes Bioconversion Amino Acids Reduction Chemistry Eukaryotes Hyperoxia Bacteria Photosynthesis Organic Compounds Glutathione Ntrs Nasa Technical Reports Server (ntrs) Nasa Technical Reports Server Aerodynamics Aircraft Aerospace Engineering Aerospace Aeronautic Space Science |
| Content Type | Text |
| Resource Type | Article |
