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Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4
| Content Provider | Hyper Articles en Ligne (HAL) |
|---|---|
| Author | Kubac, David Kaplan, Ondrej Elisakova, Veronika Patek, Miroslav Vejvoda, Vojtech Slamova, Kristyna Tothova, Andrea Lemaire, Marielle Gallienne, Estelle Lutz-Wahl, Sabine Fischer, Lutz Kuzma, Marek Pelantova, Helena Pelt, Sander Van Bolte, Jean Kren, Vladimir Martinkova, Ludmila |
| Abstract | A semi-purified nitrile hydratase from Rhodococcus erythropolis A4 was applied to biotransformations of 3-oxonitriles 1a–4a, 3-hydroxy-2-methylenenitriles 5a–7a, 4-hydroxy-2-methylenenitriles 8a–9a, 3-hydroxynitriles 10a–12a and 3-acyloxynitrile 13a into amides 1b–13b. Cross-linked enzyme aggregates (CLEAs) with nitrile hydratase and amidase activities (88% and 77% of the initial activities, respectively) were prepared from cell-free extract of this microorganism and used for nitrile hydration in presence of ammonium sulfate, which selectively inhibited amidase activity. The genes nha1 and nha2 coding for α and β subunits of nitrile hydratase were cloned and sequenced. |
| Ending Page | 113 |
| Page Count | 7 |
| Starting Page | 107 |
| File Format | |
| ISSN | 13811177 |
| Journal | Journal of Molecular Catalysis B: Enzymatic |
| Volume Number | 50 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-01-01 |
| Access Restriction | Open |
| Subject Keyword | Rhodococcus erythropolis Nitrile hydratase Amidase Branched and substituted nitriles Cross-linked enzyme aggregates chim Chemical Sciences Organic chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Catalysis Bioengineering Process Chemistry and Technology |
