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| Content Provider | frontiers |
|---|---|
| Author | Bernardo, Wagner Luis de Carvalho Boriollo, Marcelo Fabiano Gomes Tonon, Caroline Coradi da Silva, Jeferson Júnior Oliveira, Mateus Cardoso de Moraes, Fernando Cruz Spolidorio, Denise Madalena Palomari |
| Abstract | This study analyzed the antimicrobial and antibiofilm action and cytotoxicity of extract (HEScL) and silver nanoparticles (AgNPs-HEScL) from Syzygium cumini leaves. GC-MS, UV-Vis, EDX, FEG/SEM, DLS and zeta potential assays were used to characterize the extract or nanoparticles. Antimicrobial, antibiofilm and cytotoxicity analyses were carried out by in vitro methods: agar diffusion, microdilution and normal oral keratinocytes spontaneously immortalized (NOK-SI) cell culture. MICs of planktonic cells ranged from 31.2-250 (AgNPs-HEScL) to 1,296.8-10,375 g/ml (HEScL) for Actinomyces naeslundii, Fusobacterium nucleatum, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus oralis, Veillonella dispar, and Candida albicans. AgNPs-HEScL showed antibiofilm effects (125-8,000 g/ml) towards Candida albicans, Streptococcus mutans and Streptococcus oralis, and Staphylococcus aureus and Staphylococcus epidermidis. The NOK-SI exhibited no cytotoxicity when treated with 32.8 and 680.3 g/ml of AgNPs-HEScL and HEScL, respectively, for 5 min. The data suggest potential antimicrobial and antibiofilm action of HEScL, and more specifically, AgNPs-HEScL, involving pathogens of medical and dental interest (dose-, time- and species-dependent). The cytotoxicity of HEScL and AgNPs-HEScL detected in NOK-SI was dose- and time-dependent. This study presents toxicological information about the lyophilized ethanolic extract of S. cumini leaves, including their metallic nanoparticles, and adds scientific values to incipient studies found in the literature. |
| ISSN | 1664302X |
| DOI | 10.3389/fmicb.2022.995521 |
| Volume Number | 13 |
| Journal | Frontiers in Microbiology |
| Language | English |
| Publisher Date | 2022-09-29 |
| Access Restriction | Open |
| Subject Keyword | Syzygium cumini Antibiofilm activity Silver nanoparticles Dental pathogens Citotoxic activity |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
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