NDLI: Combining Mass Spectrometry and X-Ray Crystallography for Analyzing Native-Like Membrane Protein Lipid Complexes

Content Provider	frontiers
Author	Montenegro, Felipe A. Cantero, Jorge R. Barrera, Nelson P.
Abstract	Membrane proteins represent a challenging family of macromolecules, particularly related to the methodology aimed at characterizing their three-dimensional structure. This is mostly due to their amphipathic nature as well as requirements of ligand bindings to stabilize or control their function. Recently, Mass Spectrometry (MS) has become an important tool to identify the overall stoichiometry of native-like membrane proteins complexed to ligand bindings as well as to provide insights into the transport mechanism across the membrane, with complementary information coming from X-ray crystallography. This perspective article emphasizes MS findings coupled with X-ray crystallography in several membrane protein lipid complexes, in particular transporters, ion channels and molecular machines, with an overview of techniques that allows a more thorough structural interpretation of the results, which can help us to unravel hidden mysteries on the membrane protein function.
ISSN	1664042X
DOI	10.3389/fphys.2017.00892
Volume Number	8
Journal	Frontiers in Physiology
Language	English
Publisher Date	2017-11-09
Access Restriction	Open
Subject Keyword	Molecular dynamics Native mass spectrometry Ligand binding Membrane protein Mass Spectrometry Gas phase
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical)

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Combining Mass Spectrometry and X-Ray Crystallography for Analyzing Native-Like Membrane Protein Lipid Complexes