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| Content Provider | frontiers |
|---|---|
| Author | Zhao, Tanjun Ren, Liyuan Li, Chengda Liu, Li Zou, Yang Yan, Hanyu Zhan, Yaoyao Chang, Yaqing |
| Abstract | MicroRNA-7 (miR-7) is a highly conserved short non-coding RNA involved in various bioprocesses via the regulation of multiple target genes. To enrich our knowledge of the functions of miR-7 in innate immune regulation in echinoderms, we first investigated the targeting relationship between miR-7 and PAK1 in the sea cucumber Apostichopus japonicus and then explored the functions of miR-7, the PAK1 gene, and the miR-7/PAK1 axis in the pathogen-induced immune response of A. japonicus. Our results showed that miR-7 can bind to the 3ʹUTR of PAK1 and negatively regulate the expression of PAK1 in A. japonicus. Overexpression and inhibition of miR-7 and inhibition of the expression of PAK1 can alter phagocytosis, cellular agglutination, and lysozyme contents in A. japonicus. Both miR-7 and the PAK1 gene are involved in immune defense against Vibrio splendidus infection; the miR-7/AjPAK1 axis showed immune regulatory function at 48 to 72 h post-infection (hpi) after V. splendidus infection in A. japonicus. In summary, the results of this study established that miR-7 regulates the pathogen-induced immune response by targeting PAK1 in A. japonicus. |
| ISSN | 16643224 |
| DOI | 10.3389/fimmu.2022.927796 |
| Volume Number | 13 |
| Journal | Frontiers in Immunology |
| Language | English |
| Publisher Date | 2022-07-14 |
| Access Restriction | Open |
| Subject Keyword | MiR-7 Apostichopus japonicus Innate immune regulation Vibrio splendidus Pak1 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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