Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | frontiers |
|---|---|
| Author | Joshi, Amaraja Thite, Sonia Karodi, Prachi Joseph, Neetha Lodha, Tushar |
| Abstract | A Gram-stain positive, long, rod-shaped, motile and spore-forming bacterium (MEB199T) was isolated from a sediment sample collected from Lonar lake, India. The strain was oxidase- and catalase-positive. The strain grew optimally at pH 10, NaCl concentration of 3.5 % at 37 C. The major fatty acid was iso-C15:0, iso-C16:0, anteiso-C15:0, and iso-C17:0. The peptidoglycan contained meso-diaminopimelic acid. Phosphatidylethanolamine, diphosphatydilglycerol and phosphatidylglycerol, were the major polar lipids of MEB199T. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain MEB199T belonged to the family Bacillaceae and exhibited a distinctive position among the members of the genus Alkalihalobacillus. Strain MEB199T shared the highest 16S rRNA gene sequence similarity with Alkalihalobacillus alkalinitrilicus ANL-iso4T (98.36 %), whereas with type species A. alcalophilus DSM 485T it is 94.91 %, indicating that strain MEB199T distinctly related to the genus Alkalihalobacillus. The G+C content of genomic DNA was 36.47 mol %. The dDDH (23.6 %) and average nucleotide identity (81 %) values between strain MEB199T and Alkalihalobacillus alkalinitrilicus ANL-iso4T confirmed the novelty of this new species. The different phylogenetic analysis based on the genome showed that the members of the genus Alkalihalobacillus separated into seven distinct clades. The intra-clade average amino acid identity and percentage of conserved proteins range from 52-68 % and 37-59 %, respectively which are interspersed on the intra-genera cut-off values, therefore we reassess the taxonomy of genus Alkalihalobacillus. The phenotypic analysis also allows corroborated the differentiation between these clades. Based on the phylogenetic analysis, genomic indices and phenotypic traits we propose the reclassification of the genus Alkalihalobacillus into seven new genera for which the names Alkalihalobacterium gen. nov., Halalkalibacterium gen. nov., Halalkalibacter gen. nov., Shouchella gen. nov., Pseudalkalibacillus gen. nov., Alkalicoccobacillus gen. nov., Alkalihalophilus gen. nov. are proposed and provide an emended description of Alkalihalobacillus sensu stricto. Also, we propose the reclassification of Alkalihalobacillus okuhidensis as a sub-species of Alkalihalobacillus halodurans. Based on the polyphasic taxonomic analysis, strain MEB199T represent a novel species of newly proposed genus for which the name Alkalihalobacterium elongatum gen. nov. sp. nov. is proposed. The type strain is MEB199T (=MCC 2982T, =JCM 33704T, =NBRC 114256T, =CGMCC 1.17254T). |
| ISSN | 1664302X |
| DOI | 10.3389/fmicb.2021.722369 |
| Volume Number | 12 |
| Journal | Frontiers in Microbiology |
| Language | English |
| Publisher Date | 2021-10-11 |
| Access Restriction | Open |
| Subject Keyword | Lonar lake Alkaliphilic bacteria Alkalihalobacterium Alkalihalobacillus Taxogenomic |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Microbiology (medical) |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|